Effect of chitosan interferon-γ nanogene (CIN) therapy on the dendritic cell population in the lung. Ovalbumin-allergic/-challenged mice were treated with or without CIN therapy as described in Materials and Methods, and mononuclear cells were isolated from the lungs. Cells were stained with fluorescein isothiocyanate (FITC)-anti-CD11c and rhodamine (Rhod)-anti-CD11b, and CD11c+b+ cells were counted by flow cytometry. Values given are the percentage of total CD11c+ cells that are also CD11b+. The data are based on cytometry of a minimum of 15,000 cells and were substantiated by a repeat experiment.
Kong et al. Allergy, Asthma & Clinical Immunology 2008 4:95 doi:10.1186/1710-1492-4-3-95