Figure 3.

Effect of chitosan interferon-γ nanogene (CIN) therapy on the dendritic cell population in the lung. Ovalbumin-allergic/-challenged mice were treated with or without CIN therapy as described in Materials and Methods, and mononuclear cells were isolated from the lungs. Cells were stained with fluorescein isothiocyanate (FITC)-anti-CD11c and rhodamine (Rhod)-anti-CD11b, and CD11c⁺b⁺ cells were counted by flow cytometry. Values given are the percentage of total CD11c⁺ cells that are also CD11b⁺. The data are based on cytometry of a minimum of 15,000 cells and were substantiated by a repeat experiment.